Fig. 3.

The ouabain effect on NMDAR-mediated calcium response is not due to internalization of NMDAR or Src-activation. a Comparison between cytosolic and membrane expression of NMDAR in the presence of NMDA (10 μM) alone and with ouabain (10 nM). The endocytotic index was measured in cells treated for 5 min and 15 min. One-way ANOVA and Bonferroni’s post hoc test were used. b Traces of calcium responses in cells treated for 20 s with NMDA (10 mΜ) alone and with ouabain (10 nM), in the presence of PP2 (1 μM). c Boxplot presentation over ΔF/F0 upon stimulation with NMDA and respective NMDA + ouabain. ***p < 0.001, Wilcoxon signed-rank test