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. 2020 Jul 10;57(10):4018–4030. doi: 10.1007/s12035-020-01984-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Proximity ligation assay (PLA) images of rat hippocampal neurons. PLA was performed using antibodies against Na,K-ATPase α-subunits (NKAα1 and NKAα3) and against GluN2-subunits (GluN2A and GluN2B). Omission of primary antibodies was used as negative control, and antibodies against GluN1 was used as a positive control. Red dots indicate PLA signal; cell nuclei are identified using DAPI stain. Upper left image is PLA of NKAα3 with GluN2B counterstained with pan neuronal marker in green to visualize neurons with extensions. Scale bar = 50 μm