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. 2020 Sep 2;11:4399. doi: 10.1038/s41467-020-18133-0

Fig. 5. TOR pathway is required for growth and tentacle budding in fed polyps.

Fig. 5

a Confocal z-section of the oral pole of unfed polyp stained with with an antibody against pRPS6 (red) and Hoechst to label nuclei (white) in four independent experiments (n = 28 polyps). Inset box is a zoom in of the segment 3. b Confocal sections of developing buds at sequential stages (n = 32 polyps). Inset boxes show a higher magnification of segment 3 for each stage. Dashed lines separate the outer- and inner-layer nuclei. Arrows indicate the enrichment of pRPS6 in the inner-layer of tentacle primordia. c Confocal projection of the oral pole of 5 days fed control and 8 days fed rapamycin-treated polyps stained for EdU incorporation (green), an antibody against pRPS6 (red) and with Hoechst (white; three independent experiments). White arrowhead indicates an unspecific EdU-labeling in the pharynx. d Plots of EdU intensity in control polyps (n = 5) and rapamycin-treated polyps (n = 5). Scale bars are 50 μm. e Quantification of polyps showing PS6RP-postive tentacle primordia in control polyps (n = 25) and rapamycin-treated polyps (n = 26). Data are mean ± SD for error bars (unpaired Student’s two-tailed t-test, ****p < 0.0001). Source data are provided as a Source data file.