Fig. 3.

Retrospective analysis of developmental competence from morulae to blastocysts in the clinical data set. The rates of development from morulae to blastocysts (a) and to form morphologically good blastocysts (b) were compared retrospectively between two maternal age groups (< 37 years; n = 280; vs. ≥ 37 years, n = 251). Retrospective analysis revealed that the rates of development from morulae to blastocysts and to morphologically good blastocysts on day 5 after insemination decreased with maternal age. The rates of development from morulae to blastocysts and to morphologically good blastocysts on day 6 also decreased with maternal age. The data were for embryos cultured in two different commercially-available media (see Methods). Similar adverse effects of maternal age were observed in both culture media (Supplementary Figure)