Table 1. Amino acid sequences of the foot-and-mouth disease virus (FMDV) type O VP1 GH loop epitope insertion site within the vesicular stomatitis virus glycoprotein and glutathione S-transferase.
Group | Amino acid sequence (N to C) |
---|---|
VSV GP-VP1a) | GP-WIVYNGNCKYTGGSLPNVRGDLQVLAPKAARCLPTSFNYGAIKDP-162 |
GST-VP1b) | GST-VYNGNCKYTGGSLPNVRGDLQVLAPKAARCLPTSFNYGAIK |
Underlined sequences indicate VP1 129 to 169 amino acid residues from FMDV (GenBank: KX162590) isolated in 2014, South Korea, with displacement of proline to cysteine at residue 158 to enhance neutralization activity [13]. a) Recombinant vesicular stomatitis virus (VSV) glycoprotein fused to FMDV VP1 epitope (129–169). b) Recombinant glutathione S-transferase (GST) fused to FMDV VP1 epitope (129–169).