Figure 2. miR-4454 affected the invasion and migration on HPV16 E6/E7-positive CaSki cells.

(A) Transwell assay was used to detect cell invasion in CaSki and C33A cells. (B) Statistical analysis of the invasion of human cervical cancer cells (CaSki and C33A) treated with miR-4454 mimics and miR-4454 inhibitor. (C) Transwell assay was used to detect cell migration in CaSki and C33A cells. (D) Statistical analysis of migration in human cervical cancer cells (CaSki and C33A) treated with miR-4454 mimics and miR-4454 inhibitor. All data were obtained in triplicate; *, **, and *** indicate P<0.05, P<0.01, and P<0.001, respectively. (E) Interactions between miR-4454 and ABHD2 evaluated in 293 cells by a dual luciferase assay. (F) Relative mRNA expression levels of ABHD2 in cells transfected with the miR-4454 mimics and miR-4454 inhibitor. (G) Relative expression levels of miR-4454 in cells transfected with the pre-ABHD2 and sh-ABHD2. (H) Interactions between miR-4454 and NUDT21 evaluated in 293 cells by a dual luciferase assay. (I) Relative mRNA expression levels of NUDT21 in cells transfected with the miR-4454 mimics and miR-4454 inhibitor. (J) Relative expression levels of miR-4454 in cells transfected with the pre-NUDT21 and sh-NUDT21. (K) Western blot analyses of ABHD2, NUDT21, Caspase 3, and Cleaved Caspase 3 in C33A cells transfected with miR-4454 mimics and miR-4454 inhibitor. (L) Relative protein expression levels of ABHD2, NUDT21, Caspase 3, and Cleaved Caspase 3 in C33A cells transfected with the miR-4454 mimics and miR-4454 inhibitor. (M) Western blot analyses of ABHD2, NUDT21, Caspase 3, and Cleaved Caspase 3 in CaSki cells transfected with miR-4454 mimics and miR-4454 inhibitor. (N) Relative protein expression levels of ABHD2, NUDT21, Caspase 3, and Cleaved Caspase 3 in CaSki cells transfected with the miR-4454 mimics and miR-4454 inhibitor. Data were obtained in triplicate; *, **, and *** indicate P<0.05, P<0.01, and P<0.001, respectively.