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. 2020 Sep 2;11:4411. doi: 10.1038/s41467-020-18115-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Schematic of experiment. b Representative Cy5 images of the femoral vein from animals 90 min after evans blue (EB) injection during the day (orange) or night (blue). White scale bar: 1 mm. c Mean pixel intensity in arbitrary units (A.U.) over 90 min post EB injection. Thick lines indicate group means with SEM shaded, individual points indicate time image was taken. The inset is a boxplot of the magnitude of fluorescence at 90 min, two-sided t test, p = 0.0256. Time 0 is tracer infusion start. Day: n = 9 mice, night: n = 6 mice. Asterisk indicates p < 0.05. d Schematic of processing brains to check injection localization and specificity. e Representative striatal EB injection sites. Blue circle indicates probe end. White scale bar: 1 mm. f Boxplot of percent area of EB quantified in a subset of brains during the day (n = 6 mice) and night (n = 4 mice). All boxplots: minima is minimum value, maxima is maximum value, center is median and quartiles shown by box and whiskers, with individual animals shown as colored dots, ns: not significant. Source data are provided as a Source Data file.