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. 2020 Sep 2;10:14492. doi: 10.1038/s41598-020-71171-y

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© Crown 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PSD-95 protein levels are increased in the striatum of NL3^R451C mice, whereas PSD-93 and NL2 expression is unchanged. Levels of postsynaptic proteins, PSD-95, PSD-93 and NL2 in cortical (A,G,M), striatal (C,I,O) and cerebellar (E,K,Q) brain lysates from NL3^R451C and WT mice were analysed via Western blot. Densitometric analysis was performed to demonstrate quantitative expression of PSD-95 (B,D,F), PSD-93 (H,J,L) and NL2 (N,P,R) relative to β actin expression. Genotype differences were analysed using an unpaired, two-tailed Student’s t-test (n = 6 mice in each group); *p < 0.05. Data represented as mean ± SEM.