Table 5.
Antibodies used in Western blot analysis.
| Primary antiserum | Supplier | Dilution | Secondary antiserum | Supplier | Dilution |
|---|---|---|---|---|---|
| Rabbit polyclonal anti-NL3 (110 kDa) | Synaptic systems #129113 | 1:500 | Goat anti-rabbit IgG HRP | Dako #P0048 | 1:1,000 |
| Rabbit polyclonal anti-PSD-93 (110 kDa) | Alomone Labs #APZ002 | 1:500 | |||
| Rabbit polyclonal anti-NL2 (91 kDa) | Synaptic systems #129203 | 1:500 | |||
| Rabbit polyclonal anti-SYT-1 (48 kDa) | Alomone Labs #ANR-003 | 1:500 | |||
| Rabbit polyclonal anti-GFAP (48 kDa) | Dako #Z0334 | 1:5,000 | |||
| Rabbit polyclonal anti-SNAP-25 (23 kDa) | Alomone Labs #ANR-001 | 1:500 | |||
| Mouse monoclonal anti-PSD-95 (95 kDa) | UC Davis/NIH NeuroMab Facility #K28/43 | 1:5,000 | Goat anti-mouse IgG HRP | Dako #P0447 | 1:1,000 |
| Mouse monoclonal anti-β-actin (42 kDa) | Sigma-Aldrich #A5441 | 1:4,000 |