Fig. 10. sNuc-seq analysis reveals two transcriptionally distinct populations of SCNVIP neurons.
a Schematic of method for labeling, isolating, and profiling SCNVIP neuron transcriptomes for sNuc-Seq; images are from samples used in this study. b t-Distributed Stochastic Neighbor Embedding (tSNE) plot of 144 VIP SCN neurons (dots) profiled by sNuc-Seq and clustered according to their expression of 689 high-dispersion genes; colors indicate cluster identity. c Heatmap showing single-cell (column) expression of top cluster marker genes (rows), with cells (columns) binned by cluster identity. d Violin plots of single-cell expression values of Vip and selected cluster marker genes. See also Fig. S11.