Fig. 4. Cell-type specific ablation of SCNVIP neurons, but not SCNAVP neurons, reduces the amplitude of the sleep-wake circadian rhythm.
a Hourly amounts (mean ± s.e.m.) of wakefulness (a1), NREM sleep (a2), and REM sleep (a3) in AVP-IRES-Cre (AVPDTA; gray, n = 6 mice) and VIP-IRES-Cre (VIPDTA; gray, n = 6 mice) that received bilateral injections of DTA vector into the SCN, during the 4th and 5th day in constant darkness. b Upper panels: Amounts (mean ± s.e.m.) of the vigilance stages during the 4th and 5th subjective dark (SD4, SD5) and 4th and 5th subjective light (SL4, SL5) periods. Lower panels: 24 h amounts (mean ± s.e.m.) of the vigilance stages during the 4th and 5th day in constant darkness (DD4, DD5; lower left panels) and subjective night to subjective day (D/L) ratio for each vigilance stage during the 4th and 5th day in constant darkness (lower right panels). n = 6 mice per group,*p < 0.05, **, two-way ANOVA followed by a post hoc Bonferroni test. See also Figs. S7 and S8.