FIG 1.

Proposed overall structure of LPS fragments. Proposed structures of LPS fragments from P. gingivalis (A) and T. forsythia (C) in their native contexts bonded to a cargo protein. The major reaction products after cleavage with proteinase K and deglycosylation with TFMS in the presence of toluene are shown for P. gingivalis (B) and T. forsythia (D). The position of the glycosidic bonds (bonds between sugars) are unknown except for the C-1 positions as shown (A and C). The glycosidic bond position shown in the reaction product (B and D), indicated with a star, is only one of several possibilities. The three components, I, II, and III, are indicated by dashed boxes and represent the major CID fragments observed. For accurate mass data for the determination of molecular formulae, see the main text and Tables 2 and 3.