FIG 2.

Deglycosylation in the presence of ethylbenzene demonstrates aryl adducts. Orbitrap LC-MS/MS analysis of a modified RgpB C-terminal peptide with the sequence VEGT after deglycosylation in the presence of ethylbenzene (EB) instead of toluene. The mass of component III was increased by 28 Da (compare with value shown in Fig. 1B), suggesting the presence of two EB molecules and a hexose residue. The arrow indicates that the mass difference of 374 Da is relative to the precursor m/z of 1,063.52.