FIG 8.

Hybrid cells in the coculture of red C. acetobutylicum-Halo and WT C. ljungdahlii labeled green with SYTO RNASelect dye. The red signal came from Janelia Fluor 646 ligand bound to the HaloTag in C. acetobutylicum-Halo strain. The green signal came from RNASelect dye bound to RNA in C. ljungdahlii cells. Images were collected at different coculture time points in Z-stack mode using SR-SIM. (A) A single hybrid cell observed at 4 h of coculture. The cell contains red protein and green RNA, which are uniformly distributed throughout the cell. (B) A single hybrid cell observed at 20 h of coculture. The cell contains both red and green fluorescent material, and displays the clostridial sporulation form associated with the sporulation mechanism in C. acetobutylicum.