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. 2020 Sep 2;10:14437. doi: 10.1038/s41598-020-71474-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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dGRAPHIC application in Xenopus blastomere. (A) Schema of mRNA injection into 8-cell-stage Xenopus blastomeres. The mRNA of the NT probe is injected in the right animal-smaller blastomere of the Xenopus 8-cell stage, and the mRNAs of the CT probe and nuc-mCherry are injected together into the left animal-smaller blastomere. (B) Embryos are grown to the early neurula stage. Cells expressing the CT probe (red nuclei) are present only within the left half of the neural plate, while GFP signals can be detected only in the right half of the neural plate. Scale bar, 100 μm.