Fig. 2.
The discovery of compound DC_CP20 and hits validation. a Flowchart of hierarchical high-throughput screening for CBP BrD inhibitors. b Inhibitory activity of top 26 compounds from high-throughput screening determined by TR-FRET assay. c Structure of compound DC_CP20. d IC50 determination for compound DC_CP20 against CBP BrD by TR-FRET assay. e SPR-binding data for the interactions of DC_CP20 with CBP BrD. The compound was prepared at concentration of 0.173, 0.390, 0.585, 0.878, 1.975, 2.963, 4.444, and 6.667 μM, respectively