Table 2.
Summary of crystallization procedures.
| PDB code | Method/component | Crystallization solution | Conformation of ADP-ribose |
|---|---|---|---|
| 6QZU |
Crystallization GETV macro domain 16 mg·mL−1 |
0.2 M imidazole/malate pH 6.0, 30% PEG 4 K | |
| 6R0F |
Co-crystallization GETV macro domain 13 mg·mL−1, 3 mM ADP-ribose Soacking 15 mM aspartic acid |
0.2 M imidazole/malate pH 5.9, 34% PEG 4 K | ADP-ribose with closed distal ribose, “pose 1” |
| 6R0G |
Co-crystallization GETV macro domain 13 mg·mL−1, 3 mM ADP-ribose, 50 mM glutamic acid |
0.2 M imidazole/malate pH 6.0, 30% PEG 4 K | ADP-ribose with closed distal ribose, “pose 2” |
| 6R0T |
Co-crystallization GETV macro domain 13 mg·mL−1, 3 mM ADP-ribose + 30 mM aspartic acid |
0.2 M imidazole/malate pH 5.9, 34% PEG 4 K | ADP-ribose with open distal ribose |
| 6R0P |
Co-crystallization GETV macro domain 13 mg·mL−1, 3 mM ADP-ribose, 30 mM aspartic acid |
0.2 M imidazole/malate pH 6.0, 32% PEG 4 K | ADP-ribose with open distal ribose in double conformation |
| 6R0R |
Co-crystallization GETV macro domain 13 mg·mL−1, 3 mM ADP-ribose, 3 mM aspartic acid |
0.2 M imidazole/malate pH 5.9, 38% PEG 4 K | ADP-ribose with distal ribose covalently linked to Cys34 |