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. 2020 Sep 2;11(8):718. doi: 10.1038/s41419-020-02931-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Swine SK6 cells were mock-transfected or transfected with 2 µg of a plasmid encoding DDK-MDA5 and 24 h later infected with type-O or type-C FMDV at an MOI of 5. Cells were lysed at different times after infection. a Lysates were analyzed by immunoblot for detection of the indicated proteins using the specified antibodies. Arrows indicate the N- and C-terminal cleavage products of MDA5. The 110-kDa cleavage product of eIF4G is also depicted. b Viral titers in supernatants collected from cells analyzed in a (infected with type-O virus) were determined by plaque assay on IBRS-2 cells. Data as mean of triplicates ± SD. c Antiviral activity in supernatants from cells analyzed in a (infected with type-O virus) is expressed as the reciprocal of the highest dilution of the supernatant needed to reduce the number of VSV plaques on IBRS-2 cells by 50%. When indicated, supernatants were previously treated with a monoclonal antibody anti-swine IFN-α. Data are average of three independent assays ± SD.