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. 2020 Jan 16;41(6):753–762. doi: 10.1038/s41401-019-0347-0

Fig. 9. The affinity constant of DTIP and mutants.

Fig. 9

DTIP (0.01 mg/mL) and mutant variants (0.01 mg/mL) were labeled with biotin for about 1 h. Thrombin was diluted to different concentrations from 8.64 μmol/L to 0.27 μmol/L. Binding was proceeded with the software of ForteBio Octet RED 96. Each group was given five concentration gradients. a The binding of thrombin to DTIP, KD = 1.64 × 10−7 M, R2 = 0.9976. b the binding of thrombin to Y3A, KD = 1.62 × 10−7 M, R2 = 0.9936. c The binding of thrombin to C6A-C14A, KD = 1.38 × 10−7 M, R2 = 0.9924. d The binding of thrombin to D24A, KD = 1.94 × 10−7 M, R2 = 0.9957. e The binding of thrombin to E35A, KD = 1.88 × 10−7 M, R2 = 0.9945. f No binding of thrombin with enoxaparin sodium.