Table 2.
Various parameters in the BLI
| Samples |
KD (M) |
KD error |
Kon [1/(M·s)] |
Kon error |
Kdis (1/s) |
Kdis error |
Full X2 |
Full R2 |
|---|---|---|---|---|---|---|---|---|
| DTIP | 1.64 × 10−7 | 2.25 × 10−9 | 2.56 × 103 | 14.3 | 4.18 × 10−4 | 5.25 × 10−6 | 2.5441 | 0.9976 |
| Y3A | 1.62 × 10−7 | 1.86 × 10−9 | 3.76 × 103 | 22.1 | 6.09 × 10−4 | 6.00 × 10−6 | 9.8732 | 0.9936 |
| C6A-C14A | 1.38 × 10−7 | 2.56 × 10−9 | 2.98 × 103 | 20.7 | 4.10 × 10−4 | 7.06 × 10−6 | 4.7585 | 0.9924 |
| D24A | 1.94 × 10−7 | 1.76 × 10−9 | 3.40 × 103 | 16.6 | 6.59 × 10−4 | 5.04 × 10−6 | 6.623 | 0.9957 |
| E35A | 1.88 × 10−7 | 1.90 × 10−9 | 3.38 × 103 | 18.0 | 6.35 × 10−4 | 5.46 × 10−6 | 9.6834 | 0.9945 |
Equilibrium dissociation constant (KD): the degree of affinity
Association rate constant (Kon): rate of product formation per unit time
Dissociation rate constant (Kdis): percentage of product degradation per unit time