Fig. 4.

Effects of STAT3 siRNA on ZYZ-803-induced angiogenesis in HUVECs. a Knockdown of STAT3 inhibited ZYZ-803-induced STAT3 phosphorylation, but upregulated the expression of CaMKII. HUVECs were treated with ZYZ-803 (1 µM) for 30 min after STAT3 silencing, which was performed by transfection using an RNAiMAX kit for 48 h. b The mRNA level of STAT3 was quantified using quantitative RT-PCR. HUVECs were treated with ZYZ-803 (1 µM) for 30 min after transfection with siRNA (STAT3) for 48 h. c For proliferation assay, transwell migration assay as well as wound-healing assay, HUVECs were treated with ZYZ-803 (1 µM) for 24 h, for tube formation assay, cells were treated with ZYZ-803 (1 µM) for 6 h after transfection with siRNA (STAT3) for 48 h. Scale bars, 100 μm. n = 3 independent experiments. *P < 0.05, **P < 0.01 versus control; ^#P < 0.05, ^##P < 0.01 versus ZYZ-803 treatment group