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. 2020 Sep 3;286:113965. doi: 10.1016/j.jviromet.2020.113965

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Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

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Fig. 1 — (A) Heat map of SARS-CoV-2 gene equivalents (log10) for 56 COVID-19 patients using a commercial RNA isolation kit or COVID-quick-DET. The colors in the heat map represent gene expression levels (log10). White crossed boxes marked with “#” represent the samples giving discrepant results between COVID-quick-DET and the reference method. Samples taken from bronchoalveolar lavages are indicated by asterisk. (B) Time for RNA isolation per 100 samples in minutes. (C) Costs for RNA isolation per 100 samples in Euro. (D) Schematic workflow of tested material compatible with COVID-Quick-DET method and successful qPCR. (E) Detection of SARS-CoV-2 ORF1b-nsp14 in samples prepared by serial dilution of virus stocks of two strains in medium (French-left panel; Dutch-right panel). Viral RNA was either extracted by Qiagen Viral RNA Mini Kit (Qiagen #52906) or prepared by the COVID-quick-DET protocol before being subjected to RT-qPCR as described (Chu et al., 2020; https://www.who.int/). For direct comparison to COVID-quick-DET, RNA was eluted in the original sample volume without concentration using Qiagen Viral RNA Mini Kit. Ct 35 was set as cut-off. Reactions have been run in duplicates, values are mean ± SD. N.d. = not detected (Ct => 35).