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. 2020 Aug 24;13:8547–8556. doi: 10.2147/OTT.S251625

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© 2020 Dang et al.

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SNHG15 mediated immunotherapy through regulating miR-141. (A) The expression of miR-141 in HGC-27 cells after transfection with SNHG15 overexpression plasmid or siRNA. (B) The expression of miR-141 in gastric cancer tissues and paracancerous tissues. (C) The expression of miR-141 in HGC-27 cells compared to normal GES-1 cells. (D) Western blotting analysis of whole cell PD-L1 expression after different treatments. (E) Surface PD-L1 expression was detected with flow cytometry. (F) Cell apoptosis was detected with flow cytometry after HGC-27 cells was transfected with SNHG15 overexpression plasmid or SNHG15 overexpression plasmid and miR-141 mimics at the same time and incubated with PBMC for another 24 hours. Mean values (±SD) were calculated from three replications. **P < 0.01, ***P < 0.001.