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. 2020 Aug 24;13:8547–8556. doi: 10.2147/OTT.S251625

Figure 4.

Figure 4

The expression of SNHG15 and PD-L1 were regulated by miR-141. (A) The potential target binding sites of PD-L1 and miR-141-3p, miR-141-3p and lncRNA SNHG15. (B) Luciferase signals (left) were measured after cells were co-transfected with miR-141 mimics and a luciferase reporter containing a fragment of the PD-L1 3ʹ-UTR harboring either the miR-141 binding site (PD-L1 WT) or a mutant (PD-L1 MUT). Luciferase signals (right) were measured after cells were co-transfected with miR-141 mimics and a luciferase reporter containing a fragment of the SNHG15 3ʹ-UTR harboring either the SNHG15 binding site (SNHG15 WT) or a mutant (SNHG15 MUT). (C) Western blotting analysis of whole cell PD-L1 expression after different treatments. (D) Surface PD-L1 expression was detected with flow cytometry. Mean values (±SD) were calculated from three replications. **P < 0.01, ***P < 0.001.