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. 2020 Aug 24;13:8547–8556. doi: 10.2147/OTT.S251625

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© 2020 Dang et al.

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miR-141 regulated HGC-27 cell immunotherapy through regulating PD-L1. (A) Western blotting analysis of whole cell PD-L1 expression after different treatments. (B) Surface PD-L1 expression was detected with flow cytometry. (C) Cell apoptosis was detected with flow cytometry after HGC-27 cells was transfected with miR-141 mimic or PD-L1 overexpression plasmid and miR-141 mimics at the same time and incubated with PBMC for another 24 hours. Mean values (±SD) were calculated from three replications. *P < 0.05, **P < 0.01, ***P < 0.001.