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. 2020 Aug 20;11:1804. doi: 10.3389/fimmu.2020.01804

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Copyright © 2020 van Balen, Jedema, van Loenen, de Boer, van Egmond, Hagedoorn, Hoogstaten, Veld, Hageman, van Liempt, Zwaginga, Meij, Veelken, Falkenburg and Heemskerk.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Significant expansion and persistence of HA-1H TCR-transduced T cells could be documented in peripheral blood and bone marrow samples of patient 007. (A) Vector -specific qPCR analysis was performed on peripheral blood and bone marrow samples at indicated time-points. Orange arrow illustrates infusion of HA-1H TCR-modified T cells. (B) EBNA-3A-B7 and BZLF1-B8 and HA1H specific T cells were stimulated with patient 007 PTLD (SLC PTLD) that was not loaded or loaded with pool of the 2 EBV peptides, LCL-MRJ (HLA-A2+, B7-, B8+, HA-1H+, BZLF1+), and LCL-IZA (HLA-A2+, B7-, B8+, HA-1H-, BZLF1+). PTLD of patient 007 was not recognized by EBV-specific T-cell populations unless they were exogenously loaded with EBV-specific peptides. This PTLD was also not recognized by HA-1H-specific T cells, because the PTLD was donor-derived and therefore HA-1H negative. PTLD sample consisted of monoclonal B cells after CD19 enrichment of PBMNC.