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. 2020 Aug 19;7:211. doi: 10.3389/fmolb.2020.00211

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Copyright © 2020 Phan and Blank.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of quenching and washing on U. maydis intracellular metabolites. (A) Metabolite leakage while quenching with SB and MeOH. (B) Metabolite leakage, (C) Relative standard deviations (RSD) of all detected peaks, and (D) phosphorylated metabolite levels when U. maydis cells were washed one (x1), two (x2), and three (x3) times with SB. Each data point represents the average of three biological replication with the error bar indicating the standard deviation. Abbreviations: 2-PG, 2-phosphoglycerate, 3-PG: 3-phosphoglycerate; 6PG, 6-phosphogluconate; DHAP, dihydroxyacetone phosphate; E4P, erythrose-4-phosphate; F6P, fructose-6-phosphate; G3P, glycerol-3-phosphate; G6P, glucose-6-phosphate; PEP, phosphoenolpyruvate; Ri5P, ribose-5-phosphate; Ru5P, ribulose-5-phosphate; and S7P, sedoheptulose-7-phosphate. Normalized peak intensities of some metabolites were multiplied for data visualization.