Fig. 2.

Quantitation of microscopic aortic atherosclerosis. a Serial paraffin sections of the aortic arch were stained with hematoxylin-eosin (HE) and elastica van Gieson (EVG) or immunohistochemically stained with monoclonal antibodies (mAbs) against either macrophages (Mφ) or smooth muscle α-actin (SMA) for smooth muscle cells (SMCs). Quantitation of intimal lesions on EVG-stained sections (b) and positively stained areas of Mφ (c) and SMC (d). ♂ male, ♀ female, Tg transgenic, Non-Tg nontransgenic. Data are expressed as the mean ± SEM. n = 7–10 for each group. *P < 0.05