Fig. 4.

Quantitation of microscopic aortic atherosclerosis. a Serial paraffin sections of the aortic arch were stained with hematoxylin-eosin (HE) and Masson’s trichrome or immunohistochemically stained with monoclonal antibodies (mAbs) against either macrophages (Mφ) or smooth muscle α-actin (SMA) for smooth muscle cells (SMCs). Quantitation of intimal lesions on EVG-stained sections (b), positively stained areas of Mφ (c) and SMC (d), and collagen content (e). Data are expressed as the mean ± SEM. n = 7–10 for each group. *P < 0.05 and **P < 0.01 versus the control