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. 2020 Aug 20;11:1254. doi: 10.3389/fpls.2020.01254

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Copyright © 2020 Khosravi, Schindele, Gladilin, Dunemann, Rutten, Puchta and Houben

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confirming the target specificity of aptamer-based CRISPR imaging. (A) Immunofluorescence staining against dCas9:2xMS2:GFP combined with telomere-specific FISH. Nuclei are counterstained with DAPI (in blue). (B) Comparing the efficiency of both types of aptamer-based CRISPR imaging with FISH. Telomeric signals based on 20 isolated nuclei per each construct after ImmunoFISH. dCas9:2xMs2:GFP and dCas9:3xPP7:GFP recognized 78% and 75% of telomere signals identified by FISH, respectively (p < 0.05).