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. 2020 Aug 20;11:1254. doi: 10.3389/fpls.2020.01254

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Copyright © 2020 Khosravi, Schindele, Gladilin, Dunemann, Rutten, Puchta and Houben

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of different promotors used for expression of dCas9 on the efficiency of telomere labeling. (A) The expression of dCas9 by PRS5A promoter resulted in the recognition of a smaller number of telomeres compared to 35S and ubiquitin promoters. The XVE inducible promoter was as efficient as ubiquitin promoter regarding the number of labeled telomeres (p < 0.05). (B) 35S promoter caused the better signal to background noise ratio (p < 0.05). Data obtained from 10 isolated nuclei per construct. Regardless of promoter type, dCas9 driven by (C) RPS5A, (D) 35S, (E) XVE could label telomeric regions in N. benthamiana.