Table 1.

Summary of MinION data from all reference samples sequenced with name, subtype and barcode. Mean coverage, mapped reads and number of nucleotide differences are shown for each individual segment. The consensus identity is calculated for all segments in %

Barcode	Sample	Subtype		PB2	PB1	PA	HA	NA	NP	MP	NS	Sum	Consensus identity %
1	R30–06	H1N1	Mean Coverage	749.9	188.3	700.9	2771.9	8756.7	7987.3	13,551.5	11,637.7	5793.025	99.98528113
			Mapped Reads	8102	1131	4059	8207	22,156	20,995	31,635	30,095	126,380
			Nucleotide Differences				1	1				2
2	R3111–07	H2N9	Mean Coverage	2250.2	1360.5	1001	4396.1	626.7	4582.6	8212.6	7477	3738.3375	99.99264057
			Mapped Reads	32,171	11,283	7372	11,560	2390	10,367	18,613	18,298	112,054
			Nucleotide Differences				1					1
3	R2555–06	H3N1	Mean Coverage	6373.3	2200.9	2080.6	929.1	1968	3874.8	5749.1	5016.4	3524.025	99.98528113
			Mapped Reads	40,737	14,924	14,166	4074	6248	9628	13,859	8970	112,606
			Nucleotide Differences		1			1				2
4	HAIV-81	H4N6	Mean Coverage	1462	3401.4	792.2	4161.2	8039.7	11,356.3	14,790.1	9955.9	6744.85	99.98528113
			Mapped Reads	18,920	20,238	6874	11,778	20,138	27,900	35,597	23,680	165,125
			Nucleotide Differences	1			1					2
5	R1612–08	H5N3	Mean Coverage	5033.4	3013.7	5260.7	1441.3	3776.2	1802.1	6314.7	5898.6	4067.5875	99.96320283
			Mapped Reads	35,383	19,354	31,659	8876	10,321	4821	14,220	12,908	137,542
			Nucleotide Differences	1	1		2	1				5
6	R617–07	H6N2	Mean Coverage	3933.5	2889.7	2995.6	4024	3403.6	3564.3	9820.9	5480.4	4514	99.99264057
			Mapped Reads	29,256	21,688	23,359	13,971	9179	8292	22,581	13,515	141,841
			Nucleotide Differences				1					1
7	R11–01	H7N7	Mean Coverage	5558.2	1201	1696.6	3737.4	3467.6	6623.9	7390.9	6832.7	4563.5375	99.98528113
			Mapped Reads	46,729	12,888	17,912	15,132	8502	16,267	18,781	16,333	152,544
			Nucleotide Differences		1	1						2
8	R249–08	H9N2	Mean Coverage	4445.5	3087.6	2260.4	1729.2	2652.5	2341.6	5403.8	6044.2	3495.6	99.98528113
			Mapped Reads	32,149	20,998	14,076	6316	7275	6366	12,914	13,244	113,338
			Nucleotide Differences			1	1					2
9	WV1677–03	H10N4	Mean Coverage	4296.7	1296.4	2896.6	3175.4	2728	3976.2	7689.3	5656	3964.325	99.9779217
			Mapped Reads	41,559	12,764	26,781	9445	8429	10,422	17,043	13,420	139,863
			Nucleotide Differences			2		1				3
10	R2675–06	H11N6	Mean Coverage	924.2	912.9	1378.1	1499.1	2260.8	4951.9	4345.1	3944	2527.0125	99.99264057
			Mapped Reads	14,361	12,470	13,834	5702	5402	12,233	10,004	9893	83,899
			Nucleotide Differences	1								1
11	R2613–06	H13N8	Mean Coverage	866.9	1040.9	1199.3	6152	5035.8	10,790.8	19,984.4	16,654.6	7715.5875	99.9779217
			Mapped Reads	9250	10,656	10,600	19,030	14,503	26,581	48,937	44,445	184,002
			Nucleotide Differences	1	1			1				3
12	Se-99	H16N3	Mean Coverage	9922	426.7	599.1	115.8	2673.5	2096	5058.4	5190.9	3260.3	99.96320283
			Mapped Reads	3843	2695	5041	393	11,992	5296	12,593	11,951	53,804
			Nucleotide Differences	1	1		1	2				5