Fig. 6.

Chronic DHM treatment alleviated DA neuron loss in Dicer cKO mice. After last behavioral tests as described in Fig. 5a, mice were sacrificed either for brain tissue dissection or for immunostaining as described in Methods. a, b TH expression was detected by anti-TH antibody using Western blot. c Immunostaining was conducted with respective antibody of TH and Iba1. Representative photomicrographs were shown at ×20 magnification (scale bars, 100 μm). d Quantitative analyses of TH-positive neurons and e Ibal-positive neurons. Data were presented as mean ± SEM. Statistical analyses were performed by one-way ANOVA. n = 3 per group (*P < 0.05, **P < 0.01; tamoxifen vs vehicle. ^#P < 0.05, ^##P < 0.01; DHM vs tamoxifen)