Fig. 3.

Mebendazole inhibits the transcriptional activity of c-Maf. a HEK293T cells were cotransfected with the HA-c-Maf, pMARE.Luci and Myc-USP5 or Flag-USP7 plasmids. After 48 h, cells were treated with MBZ in a dose-dependent manner, as indicated, for another 24 h. Cells were harvested for luciferase activity measurements and IB assays. b RPMI-8226, LP1, and U266 cells were treated with MBZ in a dose-dependent manner for 24 h, followed by total RNA extraction and RT-PCR analysis. c–g LP1 and RPMI-8226 cells were treated with MBZ at the indicated concentrations for 24 h, followed by quantitative real-time PCR to measure the expression of genes, including USP5 (c), c-Maf (d), CCND2 (e), ITGB7 (f), and ARK5 (g). *P < 0.05, **P < 0.01, ***P < 0.001