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. 2020 Aug 24;43(8):718–727. doi: 10.14348/molcells.2020.0065

Fig. 3. LncRNA CRNDE promoted cell proliferation and decreased cell apoptosis by downregulating the miR-345-5p expression in the BCP-ALL cell lines.

Fig. 3

The BCP-ALL cell lines (NALM-6 and RS4;11) were divided into four groups: mimic-NC, miR-345-5p mimic + WT-CRNDE, miR-345-5p mimic + negative control (MUT-CRNDE), and miR-345-5p mimic + blank control (NC). (A and F) The expression level of miR-345-5p was measured by qRT-PCR. (B and G) Cell viability was measured using MTT assay. *P < 0.05, # P < 0.05. (C and H) Cell proliferation was measured using the BrdU assay. (D and I) The protein levels of cl-caspase 3, T- caspase 3, cl-PARP, Bax, and Bcl-2 were measured by Western blot. Β-actin was used as an internal control. (E and J) Cell apoptosis was measured using flow cytometry. **P < 0.01, ***P < 0.001 vs mimic-NC. ## P < 0.01, ### P < 0.001 vs miR-345-5p mimic + NC.