Skip to main content
. 2020 Aug 24;43(8):718–727. doi: 10.14348/molcells.2020.0065

Fig. 4. miR-345-5p negatively regulated CREB in the BCP-ALL cell lines.

Fig. 4

(A) The CREB expression was measured by Western blot in the primary normal precursor B-cells and BCP-ALL cell lines. (B) The CREB expression was measured by qRT-PCR in the BM samples collected from patients with BCP-ALL (n = 26) and healthy controls (n = 15). (C) Correlation plot of the CREB mRNA level (x-axis) and LncRNA CRNDE expression (y-axis) in the BM samples of patients with BCP-ALL. (D) Correlation plot of the CREB mRNA level (x-axis) and miR-345-5p expression (y-axis) in the BM samples of patients with BCP-ALL. (E) The putative binding sites between miR-345-5p and CREB were forecasted by Bioinformatics software. NALM-6 cells were transfected with a dual-luciferase reporter vector containing either the CREB Mut or the CREB WT in the presence of miR-345-5p mimic or miR-345-5p inhibitor or their negative controls (mimic-NC or inhibitor-NC). Forty-eight hours later, the luciferase reporter activities were detected by dual-luciferase reporter gene assay. (F) NALM-6 cells were transfected with miR-345-5p mimic or miR-345-5p inhibitor or mimic-NC or inhibitor-NC. Forty-eight hours later, the cells were harvested. The mRNA level of CREB was measured by qRT-PCR, and the protein level of CREB was measured by Western blot. *P < 0.05, **P < 0.01, ***P < 0.001 vs control or mimic-NC or inhibitor-NC.