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. 2020 Aug 24;43(8):718–727. doi: 10.14348/molcells.2020.0065

Fig. 5. miR-345-5p overexpression inhibited cell proliferation and induced cell apoptosis by downregulating the CREB expression in the BCP-ALL cell lines.

Fig. 5

The BCP-ALL cell lines (NALM-6 and RS4;11) were divided into four groups: mimic-NC, miR-345-5p mimic, miR-345-5p mimic + pcDNA-CREB, and miR-345-5p mimic + the negative control of pcDNA-CREB (pcDNA). (A and F) The expression level of miR-345-5p was measured by qRT-PCR. (B and G) The protein levels of CREB, cl-caspase 3, T-caspase 3, cl-PARP, Bax, and Bcl-2 were measured by Western blot. Β-actin was used as an internal control. (C and H) Cell viability was measured using MTT assay. (D and I) Cell proliferation was measured using the BrdU assay. (E and J) Cell apoptosis was measured using flow cytometry. **P < 0.01, ***P < 0.001 vs mimic-NC. # P < 0.05, ### P < 0.001 vs miR-345-5p mimic + pcDNA.