Skip to main content
. 2020 Aug 24;13:8463–8474. doi: 10.2147/OTT.S258680

Figure 4.

Figure 4

FOXO1 is the target of miR-183 in EC. (A) Seed region of FOXO1 mRNA 3ʹ UTR paired with miR-183. (B) Expression level of FOXO1 in EC patients’ tumor tissues and paracancerous tissues was tested by qRT-PCR analysis. (C) mRNA level of FOXO1 in paracancerous tissues, Het-1A, TE-9, and EC9706 cells. (D) Protein level of FOXO1 in paracancerous tissues, Het-1A, TE-9, and EC9706 cells. (E) Anti-miR-183 increased the expression of FOXO1 in EC9706 and TE-9 cells at the mRNA level. (F) Anti-miR-183 increased the expression of FOXO1 in EC9706 and TE-9 cells at the protein level. (G) Effect of miR-183 mimic and anti-miR-183 on changing the luciferase activities of FOXO1 3ʹ UTR reporters in EC9706 and TE-9 cells.

Notes: Data was expressed as mean±SD. *P<0.05 vs NCO group. #P<0.05 vs paracancerous tissues. &P<0.05 vs Het-1A cell line.

Abbreviations: EC, esophageal cancer; NCO, negative control oligonucleotide; FOXO1, Forkhead box O 1; 3ʹ UTR, 3ʹ untranslated region.