Figure 7.

Knockdown of miR-183 enhanced anti-tumor effect of cisplatin on EC in vivo. (A) Anti-miR-183-EC9706 or control-EC9706 cells were inoculated subcutaneously into the BALB/c nude mice (4~5 weeks old) followed by treatment with cisplatin i.p. twice a week (2 mg/kg). Twenty-five days post-inoculation, mice were sacrificed and the tumors were resected. (B) Expression level of miR-183 in the purified tumors was detected by qRT-PCR analysis. (C) Western blot analysis was used to test the protein level of FOXO1, Bim and Noxa in the purified tumors.

Notes: Data was expressed as mean±SD. *P<0.05 vs control-EC9706 cells. ^#P<0.05 vs control-EC9706+cisplatin cells.

Abbreviations: EC, esophageal cancer; qRT-PCR, quantitative reverse transcriptase real time PCR; FOXO1, Forkhead box O 1.