Skip to main content
. 2020 Sep 3;169:112572. doi: 10.1016/j.bios.2020.112572

Fig. 5.

Fig. 5

SARS-CoV-2 antigen detection. (A) Illustration of the assay mechanism. The sample-to-answer time of this assay is 40 min. (B) Entire dynamic ranges of SARS-CoV-2 S1 protein (red squares) and SARS-CoV S1 protein (black circles) in 10 times diluted human serum. The averaged background is subtracted from all data points. The solid lines are the linear fit of the data in the log-log scale. The grey shaded area marks 3 × standard deviation of the background. The lower limit of detection (LLOD) for SARS-CoV-2 S1 protein is 0.004 ng/mL <0.01% cross reactivity was observed with SARS-CoV S1 protein. (C) Calibration curves for S1 proteins between 0.06 and 15 ng/mL. The error bars are generated from duplicate measurements. (D) Entire dynamic ranges of SARS-CoV-2 N protein (red squares) and SARS-CoV N protein (black circles) in 10 times diluted human serum. The averaged background is subtracted from all data points. The solid lines are the linear fit of the data in the log-log scale. The grey shaded area marks 3 × standard deviation of the background. The lower limit of detection (LLOD) for SARS-CoV-2 N protein and SARS-CoV S1 are 0.06 ng/mL and 1 ng/mL, respectively. (E) Calibration curves for N proteins between 0.39 and 100 ng/mL. The error bars are generated from duplicate measurements. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)