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. 2020 Aug 25;2020:2647807. doi: 10.1155/2020/2647807

Figure 8.

Figure 8

RSV inhibited the inflammasome in different cell types. (a, c, e) Representative blots of NLRP3 and caspase 1 p20 in NRCMs, CFs, and macrophages, respectively. (b, d, f) Relative expression levels of NLRP3 and caspase 1 p20 in NRCMs, CFs, and macrophages, respectively. (g, h) Flow cytometry detected immune cells (marked with CD45), macrophage (marked with F4/80), and NLRP3. (i, j, k) Quantitative analysis for CD45-positive cells, F4/80-positive cells, and double-positive (F4/80 and NLRP3) labeled cells, respectively (n = 6). Each experiment was repeated three times independently. Cells were harvested for western blot analysis after 12 h treatment. Data are presented as the mean ± SD. ∗p < 0.05 vs. the no treatment or RSV group, #p < 0.05 the LPS+RSV group vs. LPS group.