Table 2.
Primer sequences for mRNA determination
| Gene | Forward Primer | Reverse Primer |
|---|---|---|
| Cs | AGAACTCATCCTGCCTCGT | CCTGCTCCTTAGGTATCAGATTG |
| Foxo1 | CTTCAAGGATAAGGGCGACAG | AGTTCCTTCATTCTGCACTCG |
| Mdh1 | GGCACAGCCTTGGAGAAATA | CTGGATACTGGGTCGATGAATG |
| Mdh2 | GAAGGAGTCGTTGAGTGTTCTT | CTCACACCTCTCACTTCATGTT |
| Nos3/Enos | CTTGAGGATGTGGCTGTGT | TGGTCCACTATGGTCACTTTG |
| Pdk4 | CATCGCCAGAATTAAACCTCAC | GGCATTCTTGAAGAGTTCGAAG |
| Pepck/Pck1 | GCCGAGACTAGCGATGGGGGT | TGGGCACATGGTTCCGCGTC |
| Pgc1α | TGCAGCCAAGACTCTGTATG | TCAGAAAGGTCAAGTTCAGGAAG |
| Ppia | CCACTGTCGCTTTTCGCCGC | TGCAAACAGCTCGAAGGAGACGC |
| Tfam | AAACCAAAAAGACCTCGTTCAG | TTTCCAAGCCTCATTTACAAGC |
| Ubb | CCAGTGGGCAGTGATGG | GCTTACCATGCAACAAAACCT |
Cs, citrate synthase; Foxo1, Forkhead Box O-1; Mdh1, malate dehydrogenase 1; Mdh2, malate dehydrogenase 2; NOS3, nitric oxide synthase; Pdk4, pyruvate dehydrogenase kinase 4; Peck, phosphoenolpyruvate carboxykinase; Pgc1α, peroxisome proliferator-activated receptor gamma coactivator 1-alpha; TFAM, mitochondrial transcription factor A; Ppia, peptidylprolyl isomerase A; Ubb, ubiquitin b.