Table 1.
Adhesion of F. prausnitzii, B. faecis and R. intestinalis to the differentiated intestinal epithelial cells Caco-2 and HT29-MTX at different multiplicities of infection (MOIs). The percent adhesion was calculated relative to the inoculums that were added to the cells for the adhesion assay. ND, not detected. Results are represented as the mean of triplicate experiments ± SD.
| Bacterial Species | MOI | Relative Adhesion (% of Inoculum) | |
|---|---|---|---|
| Caco-2 | HT29-MTX | ||
| F. prausnitzii | 100:1 | ND | ND |
| B. faecis | 100:1 | ND | ND |
| R. intestinalis | 100:1 | ND | ND |
| F. prausnitzii | 1000:1 | 1.51 ± 0.65 | 1.32 ± 0.94 |
| B. faecis | 1000:1 | 2.49 ± 1.14 | 6.40 ± 1.46 |
| R. intestinalis | 1000:1 | 0.11 ± 0.07 | 0.06 ± 0.01 |
| F. prausnitzii | 10,000:1 | 1.07 ± 0.50 | 0.02 ± 0.00 |
| B. faecis | 10,000:1 | 4.87 ± 2.69 | 5.39 ± 2.37 |
| R. intestinalis | 10,000:1 | 0.01 ± 0.00 | 0.01 ± 0.00 |