Fig. 5.

Selected gene expression profiles for human kidney peritubular microvascular endothelial cells (HKMECs) in coculture and conditioned media culture. HKMECs showed higher upregulation of kidney-specific (left) and endothelial activation (right) genes in coculture with human kidney proximal tubule epithelial cells (HPTECs) (green) compared with in HPTEC conditioned media (red). Each plotted point represents data from an independent human donor (pooled from five replicate microculture devices per donor, with an average of three quantitative RT-PCR technical replicates). Each colored bar represents the average of three human donors. Gene expression was normalized to monoculture, which was set to 1. Statistical comparisons (as described in materials and methods) are shown for genes showing significant differences between coculture and monoculture. *P ≤ 0.05; **P ≤ 0.01. HNFB1, hepatocyte nuclear factor 1 homeobox B; AJAP1, adherens junctions-associated protein 1; KCNJ16, potassium voltage-gated channel subfamily J member 16; CNTN4, contactin 4; MMP7, matrix metalloproteinase-7; MMP10, matrix metalloproteinase-10; VCAM1, vascular cell adhesion molecule-1; ANG2, angiopoietin 2.