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. 2020 May 20;319(1):L173–L184. doi: 10.1152/ajplung.00519.2019

Fig. 4.

Fig. 4.

Glucocorticoid pathway component serum/glucocorticoid–regulated kinase 1 (SGK1) is a target of microRNAs miR-424 and miR-503. A: target prediction and sequence alignment of SGK1 with hsa-miR-424 and hsa-miR-503. B: expression profile of hsa-miR-424, hsa-miR-503, and SGK1 over time during hAT2-to-AT1-like cell transdifferentiation based on integrated analysis of microarray data; n = 3. Statistical analysis was performed with polynomial regression. hsa-mR-424 P < 0.01; hsa-miR-503 P < 0.01; SGK1 P < 0.01. C: expression profile of SGK1 over days in culture by RNA-seq analysis. Statistical analysis was performed by polynomial regression; P < 0.01. D: luciferase assay of miR-424 and 503 with SGK1. A luciferase plasmid carrying the native or mutated forms of SGK1 was transfected separately or together with synthetic hsa-miR-424 or hsa-miR-503 into 293T cells. Results are shown as relative luciferase units (RLU); n = 3. Statistical analysis was performed by 2-way ANOVA. **P < 0.01.