Fig. 3.

Human neutrophil extracellular traps (NETs) alter cytokine secretion by wild-type (wt)CFBE41o- cells. wtCFBE41o- cells were exposed to PBS, 5 μg/mL NETs, 5 μg/mL NETs + 0.5 μg/mL dornase alfa (DA), or 0.5 μg/mL DA for 18 h in triplicate wells. NETs alone were simultaneously incubated for 18 h without epithelial cells. Cytokine concentrations were measured in triplicate wells of apical supernatant by Luminex, and IL-8 concentration was confirmed by ELISA. Data were analyzed by one-way ANOVA. Each symbol represents NETs isolated from a different donor. IL-1RA, IL-1 receptor antagonist. (NET donors = 4.) *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.