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. 2020 Aug 17;12(8):2477. doi: 10.3390/nu12082477

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) NeuN immunohistochemistry (a1–a4) and F-J B histofluorescence staining (b1–b4) in the CA1 area of the PYC/sham (a1,b1), PYC/SA/sham (a2,b2), PYC/TFI (a3,b3), and PYC/SA/TFI (a4,b4) groups at 5 days after TFI. In the PYC/SA/TFI group, a few NeuN⁺ CA1 pyramidal cells (arrows) and many F-J B⁺ CA1 pyramidal cells (asterisk) are observed. SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum. Scale bar = 10 μm. (B,C) The mean numbers of NeuN⁺ (B) and F-J B⁺ cells (C) in the CA1 area. The bars indicate the means ± SEM (n = 7/group, *** p < 0.001 vs. PYC/sham group, ^### p < 0.001 vs. PYC/TFI group).