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Effect of the natural peptide network_1 (NPN_1) treatment on S6 phosphorylation. C2C12 cells were treated with (a) NPN_1 (5–500 μg/mL), (b) hydrolysate A, hydrolysate B or (c) raw material (5–500 μg/mL) for 30 min following a starvation protocol and compared to untreated cells (control) and expressed as the % of controls (one-way ANOVA analysis; * p < 0.05; ** p < 0.01; *** p < 0.001; at least three independent replicates).
