Figure 4.

HFS diet-mediated changes in protein markers of lipid metabolism are altered by botanical supplementation in skeletal muscle. Protein and gene expression were assayed in skeletal muscle by Western blotting (A) and RT-PCR (E). Western blot density quantification is depicted for protein kinase B (AKT) activation (B), AMPKα activation (C) and fold change of lipid metabolism protein markers (D). RT-PCR analysis of mRNA expression (E) indicates that transcription was not affected by diet or botanical supplementation, although cd36 mRNA expression differed between the HFS group and the PMI5011-supplemented mice. Statistical significance was set at * p < 0.05, ** p < 0.01 compared to LFD (B–D); variability is expressed as mean ± SD. n = 3 (A–D); n = 5 (E).