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(A) Cy5-oligonucleotide reporters were immobilized via the PC linker at 0.2–5X of the theoretical monolayer reaction excess (0.11–2.65 mM in ACN) considering the microfluidic device’s surface area. (B) On-chip microscopy (n= 3) and (C) fluorescence spectroscopy of released Cy5 molecules (n = 3) show saturation at 1–5X. (D) Cy5-labeled oligonucleotides (n = 3), EVs (n=5), and SKBR3 cells (n=3) were released with 88%, 91%, and 94% efficiency in 2 min, respectively.
